|
KMID : 0881720170320030193
|
|
Journal of Food Hygiene and Safety
2017 Volume.32 No. 3 p.193 ~ p.198
|
|
|
Development of Analytical Method for Determination of Baicalin, Eleutheroside E, and Ligustilide by LC-MS/MS in Raw Materials of Health Functional Foods
|
|
Keum Eun-Hee
Chung So-Young
Lee Jin-Hee
Kim Mee-Hye
|
|
|
Abstract
|
|
|
|
Scutellaria baicalensis, Eleutherococcus senticosus, and Angelica sinensis have been used as raw materials for health functional foods. This study was conducted to develop a novel method to analyze levels of baicalin (Scutellaria baicalensis), eleutheroside E (Eleutherococcus senticosus), and ligustilide (Angelica sinensis) simultaneously in health functional foods. The methanol extracted samples were analyzed and quantified via liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the positive ion mode and the negative ion mode using multiple reaction monitoring. Standard calibration curves confirmed linearity with the correlation coefficient (r2) of > 0.99 at 100-2000 μg/mL concentration range. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of 13.0~35.2 μg/L and 39.3~106.7 μg/L, respectively. The recovery results ranged between 91.4~ 109.9% at 3 different concentration levels with relative standard deviations (RSDs) less than 5%. The proposed analytical method was characterized with high accuracy and acceptable precision. The new method would be an effective tool to analyze baicalin, eleutheroside E, and ligustilide simultaneously in raw materials of health functional foods.
|
|
|
KEYWORD
|
|
|
Baicalin , Eleutheroside E , Ligustilide , LC-MS/MS , Method validation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|